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Cell-Free Synthetic Biology

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ISBN: 9783039280223 9783039280230 Year: Pages: 152 DOI: 10.3390/books978-3-03928-023-0 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: General and Civil Engineering --- Technology (General)
Added to DOAB on : 2020-01-30 16:39:46
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Abstract

Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas.

Synthetic DNA and RNA Programming

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ISBN: 9783039217342 9783039217359 Year: Pages: 188 DOI: 10.3390/books978-3-03921-735-9 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Biology
Added to DOAB on : 2019-12-09 11:49:16
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Dear Colleagues, Synthetic biology is a broad and emerging discipline that capitalizes on recent advances in molecular biology, genetics, protein and RNA engineering and omics technologies. These technologies have transformed our ability to reveal the biology of the cell and the molecular basis of disease.

Keywords

fluorescent reporter --- live cell imaging --- microRNA quantification --- optogenetics --- small molecule drug screening --- Escherichia coli --- recombinant protein production --- gene overexpression --- growth effect --- ASKA collection --- codon bias --- branched-chain amino acids --- gene ontology --- genetic code expansion --- protein kinase B --- phosphoinositide dependent kinase 1 --- phosphoseryl-tRNA synthetase --- tRNASep --- alanyl-tRNA synthetase --- class II aminoacyl-tRNA synthetase --- expanded genetic code --- lysine acetylation --- posttranslational modification --- genetic code expansion --- transfer RNA --- synthetic biology --- non-canonical amino acids --- selenocysteine --- genetic code expansion --- release factor 1 --- amber stop codon suppression --- M. jannaschii orthogonal pair --- fluorescence-based screen --- cyclic peptides --- biopharmaceuticals --- mRNA display --- yeast two hybrid --- tRNASer --- mistranslation --- anticodon --- functional conservation --- alternative amino acid and nucleotide repertoires --- alternative core cellular chemistries --- biocontainment --- genetic firewall --- genetic isolation --- orthogonal central dogma of molecular biology --- synthetic life --- xenobiology --- genome engineering --- synthetic biology --- yeasts --- Metschnikowia --- genetic tools --- DNA delivery --- CUG-Ser --- reverse polymerization --- tRNA editing --- tRNA repair --- protein engineering --- synthetic biology --- tRNA --- misacylation --- indirect tRNA aminoacylation --- AspRS --- GluRS-like --- genetic code expansion --- genome synthesis --- genome editing --- microRNA --- protein modification --- RNA metabolism --- tRNA --- synthetic biology --- unnatural amino acids --- unnatural nucleotides

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MDPI - Multidisciplinary Digital Publishing Institute (2)


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english (2)


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2020 (1)

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