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Chapter: 'Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System' from book: 3D Cell Culture: Methods and Protocols (Book chapter)

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Book Series: Methods in Molecular Biology ISSN: 1064-3745 / 1940-6029 ISBN: 9781493970193 9781493970216 Year: Volume: 1612 Pages: 13 DOI: https://doi.org/10.1007/9781493970216_15 Language: English
Publisher: Springer
Subject: Oncology
Added to DOAB on : 2017-07-19 15:44:38
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Metastasis is the main cause of cancer patient mortality. Local tumor invasion is a key step in metastatic dissemination whereby cancer cells dislodge from primary tumors, migrate through the peritumoral stroma and reach the circulation. This is a highly dynamic process occurring in three dimensions that involves interactions between tumor, stromal cells, and the extracellular matrix. Here we describe the organotypic culture system and its utility to study breast cancer cell invasion induced by cancer-associated fibroblasts. This is a three-dimensional model that reproduces the biochemical and physiological properties of real tissue and allows for investigating the molecular and cellular mechanisms involving tumor and its microenvironment, and their contribution to cancer cell invasion. This system provides a robust, accurate, and reproducible method for measuring cancer cell invasion and represents a valuable tool to improve the mechanistic understanding of the initial steps in metastasis.

Plant Competition in a Changing World

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Book Series: Frontiers Research Topics ISSN: 16648714 ISBN: 9782889452057 Year: Pages: 154 DOI: 10.3389/978-2-88945-205-7 Language: English
Publisher: Frontiers Media SA
Subject: Botany --- Science (General)
Added to DOAB on : 2017-10-13 14:57:01
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Competitiveness describes a key ability important for plants to grow and survive abiotic and biotic stresses. Under optimal, but particularly under non-optimal conditions, plants compete for resources including nutrients, light, water, space, pollinators and other. Competition occurs above- and belowground. In resource-poor habitats, competition is generally considered to be more pronounced than in resource-rich habitats. Although competition occurs between different players within an ecosystem such as between plants and soil microorganisms, our topic focusses on plant-plant interactions and includes inter-specific competition between different species of similar and different life forms and intra-specific competition.Strategies for securing resources via spatial or temporal separation and different resource needs generally reduce competition. Increasingly important is the effect of invasive plants and subsequent decline in biodiversity and ecosystem function. Current knowledge and future climate predictions suggest that in some situations competition will be intensified with occurrence of increased abiotic (e.g. water and nutrient limitations) and biotic stresses (e.g. mass outbreak of insects), but competition might also decrease in situations where plant productivity and survival declines (e.g. habitats with degraded soils).Changing interactions, climate change and biological invasions place new challenges on ecosystems. Understanding processes and mechanisms that underlie the interactions between plants and environmental factors will aid predictions and intervention. There is much need to develop strategies to secure ecosystem services via primary productivity and to prevent the continued loss of biodiversity.This Research Topic provides an up-to-date account of knowledge on plant-plant interactions with a focus on identifying the mechanisms underpinning competitive ability. The Research Topic aims to showcase knowledge that links ecological relevance with physiological processes to better understanding plant and ecosystem function.

Forest Pathology and Plant Health

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ISBN: 9783038426714 9783038426721 Year: Pages: VIII, 234 DOI: 10.3390/books978-3-03842-672-1 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Biology
Added to DOAB on : 2018-01-24 13:57:31
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Every year, a number of new forest pathosystems are discovered as the result of introduction of alien pathogens, host shifts and jumps, hybridization and recombination among pathogens, etc. Disease outbreaks may also be favored by climate change and forest management. The mechanisms driving the resurgence of native pathogens and the invasion of alien ones need to be better understood in order to draft sustainable control strategies. For this Special Issue, we welcome population biology studies providing insights on the epidemiology and invasiveness of emergent forest pathogens possibly by contrasting different scenarios varying in pathogen and host populations size, genetics, phenotype and phenology, landscape fragmentation, occurrence of disturbances, management practices, etc. Both experimental and monitoring approaches are welcome. In summary, this special issue focuses on how variability in hosts, pathogens, or ecology may affect the emergence of new threats to plant species.

Coastal Morphodynamics

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ISBN: 9783038426769 9783038426752 Year: Pages: 242 DOI: 10.3390/books978-3-03842-675-2 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Geography
Added to DOAB on : 2018-01-24 14:00:59
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Every year, a number of new forest pathosystems are discovered as the result of introduction of alien pathogens, host shifts and jumps, hybridization and recombination among pathogens, etc. Disease outbreaks may also be favored by climate change and forest management. The mechanisms driving the resurgence of native pathogens and the invasion of alien ones need to be better understood in order to draft sustainable control strategies. For this Special Issue, we welcome population biology studies providing insights on the epidemiology and invasiveness of emergent forest pathogens possibly by contrasting different scenarios varying in pathogen and host populations size, genetics, phenotype and phenology, landscape fragmentation, occurrence of disturbances, management practices, etc. Both experimental and monitoring approaches are welcome. In summary, this special issue focuses on how variability in hosts, pathogens, or ecology may affect the emergence of new threats to plant species.

Diversity and environmental variability of riparian tall herb fringe communities of the order Convolvuletalia sepium in Polish river valleys

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Book Series: Monographiae Botanicae ISSN: 2392-2923 ISBN: 9788395412325 Year: Volume: 108 Pages: 130 DOI: 10.5586/mb.2019.001 Language: English
Publisher: Polish Botanical Society
Subject: Botany
Added to DOAB on : 2020-01-07 13:10:34
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The riparian tall herb fringe communities of the order Convolvuletalia sepium represent an integral part of the natural vegetation in river valleys. The major objective of this study was to assess the relationships between the diversity and variability of these communities and various environmental factors. The survey was conducted in northwestern Poland, along 101 randomly selected 1–2-km long sections of 24 rivers and the Szczecin Lagoon. Samples were collected in 2008–2013 in all types of tall herb fringe vegetation found in the surveyed river sections. Data collected included hydrogeomorphic variables, soil parameters, potential and actual vegetation, and dominant land use form. A total of 24 vegetation units were documented, based on 300 sample plots (relevés). Tall herb fringe communities occurring in valleys of large rivers (Senecionetum fluviatilis, Fallopio-Cucubaletum bacciferi, Achilleo salicifoliae-Cuscutetum lupuliformis, Convolvulo sepium-Cuscutetum europaeae typicum and chaerophylletosum bulbosi subass. nov., Rubus caesius community, Solidago gigantea community) exhibited floristic and ecological differences in comparison with plant communities from small rivers (Eupatorietum cannabini typicum, aegopodietosum and cardaminetosum amarae subass. nov., Epilobio hirsuti-Convolvuletum sepium, Soncho palustris-Archangelicetum litoralis, Convolvulo sepium-Cuscutetum europaeae aegopodietosum, Urtico-Convolvuletum sepium typicum and aegopodietosum, Urtica dioica community, Galeopsis speciosa community, Rubus idaeus community). This finding fully justified their division into two alliances: the Senecionion fluviatilis and the Archangelicion litoralis, respectively. Significant differences between the tall herb fringe communities associated with large rivers and the plant communities occurring along small rivers included plant species richness, moss layer cover, contribution of river corridor plants, level of invasion, influence of adjacent plant communities on the floristic composition, relative elevation and distance away from the riverbed, degree of shading, proportions of all grain size fractions, soil pH, contents of organic matter, humus, organic carbon, total nitrogen, bioavailable phosphorus, potassium, magnesium, and calcium in the soil. The differences in environmental characteristics of individual plant communities were significant as well; they showed that most vegetation units were well defined. The variables that best discriminated between riparian tall herb fringe communities included the natural potential vegetation unit Salici-Populetum, headwater seeps, soil pH, sample elevation above the river water level, river size, flooding, degree of shading, soil moisture, K2O and CaO contents, and C/N ratio. The integration of the main riparian gradients (longitudinal, lateral, vertical) and patch perspective (e.g., natural potential vegetation units, and land use forms) significantly improved the comprehensive riparian vegetation patterns, because these two perspectives underpin different processes shaping the vegetation. This study contributed significantly to the knowledge of riparian tall herb fringe communities. Two subassociations are described here for the first time, whereas six others have not been previously reported from Poland. The data summarized in the synoptic table indicated that the species diagnostic for individual plant communities should be revised at the supra-regional scale. Some syntaxonomic issues were also determined. The inclusion of the order Convolvuletalia sepium to the class Epilobietea angustifolii resolved the problem of classifying the community dominated by Eupatorium cannabinum, a species showing two ecological optima: one in riparian tall herb communities and the other in natural gaps of the tree stands and clearings of fertile alder carrs and riparian woodlands. This also resolved the problem of classifying the communities dominated by Galeopsis speciosa and Rubus idaeus, intermediate between riparian tall herb and clearing communities. The results of this study may serve as a reference for management of the vegetation in river valleys and promote their conservation. They may also be essential for any future syntaxonomic revision of riparian tall herb fringe communities at a larger geographical extent.

Les espèces envahissantes dans l’archipel néo-calédonien : Un risque environnemental et économique majeur

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ISBN: 9782709917759 Year: DOI: 10.4000/books.irdeditions.7612 Language: French
Publisher: IRD Éditions
Subject: Science (General)
Added to DOAB on : 2017-06-09 10:17:46
License: OpenEdition Licence for Books

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La biodiversité calédonienne, reconnue comme l’une des plus exceptionnelles avec son fort taux d’endémisme, est largement menacée par l’introduction de nouvelles espèces. Il est donc important de protéger l’écosystème calédonien, à la fois riche et vulnérable, des espèces envahissantes, l’une des principales causes de perte de la biodiversité à l’échelle mondiale. Face à cette menace, le Gouvernement et les trois provinces de Nouvelle-Calédonie souhaitent mettre en place une structure collective afin de définir les orientations stratégiques nécessaires pour préserver et conserver leur biodiversité. Elles ont ainsi voulu qu’un état des connaissances soit dressé et qu’une réflexion soit menée sur la prévention, la détection précoce, l’intervention rapide, l’éradication, le confinement et le contrôle des espèces envahissantes. Cette expertise collégiale apporte les éléments nécessaires à la construction d’un système de biosécurité pertinent et efficace pour l’archipel néo-calédonien.

Forest Insects and Pathogens in a Changing Environment: Ecology, Monitoring & Genetics (IUFRO Joint Meeting of WP7.03.05 & 7.03.10)

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ISBN: 9783039215119 / 9783039215126 Year: Pages: 158 DOI: 10.3390/books978-3-03921-512-6 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Environmental Sciences
Added to DOAB on : 2019-12-09 11:49:15
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After the successful conclusion of the Joint Meeting of IUFRO’s 7.03.05 & 7.03.10 working parties and given the exciting and novel studies that have been presented in the framework of this meeting, we decided to present some of these studies in the current Special Issue of Forests. To make this issue more appealing and interesting to everyone in the field of Forest Protection, studies that cover a wide range of topics were selected, ranging from ecology and phylogeography to forest management and protection. More importantly, as these studies refer to pests and pathogens from different parts of the world, it is expected that the knowledge gained can be further used in the protection of natural environment worldwide.

Carbonic Anhydrases and Metabolism

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ISBN: 9783038978008 9783038978015 Year: Pages: 184 DOI: 10.3390/books978-3-03897-801-5 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Biology
Added to DOAB on : 2019-04-25 16:37:17
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Carbonic anhydrases (CAs; EC 4.2.1.1) are metalloenzymes present in all kingdoms of life, as they equilibrate the reaction between three simple but essential chemical species: CO2, bicarbonate, and protons. Discovered more than 80 years ago, in 1933, these enzymes have been extensively investigated due to the biomedical application of their inhibitors, but also because they are an extraordinary example of convergent evolution, with seven genetically distinct CA families that evolved independently in Bacteria, Archaea, and Eukarya. CAs are also among the most efficient enzymes known in nature, due to the fact that the uncatalyzed hydration of CO2 is a very slow process and the physiological demands for its conversion to ionic, soluble species is very high. Inhibition of the CAs has pharmacological applications in many fields, such as antiglaucoma, anticonvulsant, antiobesity, and anticancer agents/diagnostic tools, but is also emerging for designing anti-infectives, i.e., antifungal, antibacterial, and antiprotozoan agents with a novel mechanism of action. Mitochondrial CAs are implicated in de novo lipogenesis, and thus selective inhibitors of such enzymes may be useful for the development of new antiobesity drugs. As tumor metabolism is diverse compared to that of normal cells, ultimately, relevant contributions on the role of the tumor-associated isoforms CA IX and XII in these phenomena have been published and the two isoforms have been validated as novel antitumor/antimetastatic drug targets, with antibodies and small-molecule inhibitors in various stages of clinical development. CAs also play a crucial role in other metabolic processes connected with urea biosynthesis, gluconeogenesis, and so on, since many carboxylation reactions catalyzed by acetyl-coenzyme A carboxylase or pyruvate carboxylase use bicarbonate, not CO2, as a substrate. In organisms other than mammals, e.g., plants, algae, and cyanobacteria, CAs are involved in photosynthesis, whereas in many parasites (fungi, protozoa), they are involved in the de novo synthesis of important metabolites (lipids, nucleic acids, etc.). The metabolic effects related to interference with CA activity, however, have been scarcely investigated. The present Special Issue of Metabolites aims to fill this gap by presenting the latest developments in the field of CAs and their role in metabolism.

Marine Glycosides

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ISBN: 9783038979029 9783038979036 Year: Pages: 264 DOI: 10.3390/books978-3-03897-903-6 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Medicine (General) --- Therapeutics
Added to DOAB on : 2019-05-09 17:16:14
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In recent years, there has been a steady increase in the publication of papers on the chemistry, biology, and potential clinical uses of marine glycosides. Indeed, more than half of the papers published in this field are less than a decade old. Glycosides have been isolated from species as diverse as algae, fungi, anthozoans, and echinoderms. Even fish of the genus Pardachirus produce glycosides, which they use as shark repellents.The major interest in these compounds as potential drugs stems from their broad spectrum of biological effects. They have been shown to have antimicrobial, antifungal, anti-inflammatory, immune modulatory, and anticancer effects. The anticancer effects of marine glycosides include cell cycle suppression, the induction of apoptosis, and the inhibition of migration, invasion, and metastasis, as well as antiangiogenesis. Marine glycosides influence membrane permeability and have been shown to influence membrane transport at the molecular level through effects on transport carriers and pumps, as well as effects on ligand-gated and voltage-gated channels. Various marine glycosides have been shown to activate sphingomyelinase and ceramide synthesis, to inhibit topoisomerase activity, receptor tyrosine kinase activity, and multidrug resistance protein activity, and to antagonize eicosanoid receptors.This Special Issue covers the entire scope of marine organism-derived glycosides that are of potential value as pharmaceutical agents or leads. These include, but are not limited to, tetracyclic triterpene glycosides, other triterpene glycosides, steroid glycosides, and glycosides of non-isoprenoid aglycones.

Cancer Biomarkers and Targets in Digestive Organs

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ISBN: 9783039214631 / 9783039214648 Year: Pages: 146 DOI: 10.3390/books978-3-03921-464-8 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Medicine (General)
Added to DOAB on : 2019-12-09 11:49:15
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Identification and development of cancer biomarkers and targets have greatly accelerated progress towards precision medicine in oncology. Studies of tumor biology have not only provided insights into the mechanisms underlying carcinogenesis, but also led to discovery of molecules that have been developed into cancer biomarkers and targets. Multi-platforms for molecular characterization of tumors using next-generation genomic sequencing, immunohistochemistry, in situ hybridization, and blood-based biopsies have greatly expanded the portfolio of potential biomarkers and targets. These cancer biomarkers have been developed for diagnosis, early detection, prognosis, and prediction of treatment response. The molecular targets have been exploited for anti-cancer therapy and delivery of therapeutic agents. This Special Issue of Biomedicines focuses on recent advances in the discovery, characterization, translation, and clinical application of cancer biomarkers and targets in malignant diseases of the digestive system. The goal is to stimulate basic and translational research and clinical collaboration in this exciting field with the hope of developing strategies for prevention and early detection/diagnosis of cancer in digestive organs, and improving therapeutic and psychosocial outcomes in patients with these malignant diseases.

Keywords

colorectal cancer --- intestinal disorder --- intestinal tumors --- zebrafish --- stereotactic body radiation therapy --- immunotherapy --- biomarkers --- Asian Cancer Research Group (ACRG) --- gastric carcinoma --- molecular profiling --- precision therapy --- pembrolizumab --- predictive biomarkers --- ramucirumab --- The Cancer Genome Atlas (TCGA) --- therapeutic targets --- trastuzumab --- biliary tract carcinoma --- chemotherapy --- clinical trial --- colorectal carcinoma --- gastric carcinoma --- gastrointestinal oncology --- hepatocellular carcinoma --- immunotherapy --- pancreatic carcinoma --- targeted therapy --- Liver transplantation --- liver graft injury --- intragraft gene expression profiles --- cell adhesion molecules --- CD274 --- HFE --- hepatocellular carcinoma --- immunohistochemistry --- molecular profiling --- next-generation sequencing --- precision medicine --- predictive biomarkers --- gastrointestinal oncology --- pancreatic carcinoma --- hepatocellular carcinoma --- biliary tract carcinoma --- gastric carcinoma --- colorectal carcinoma --- stereotactic body radiation therapy --- liver transplant --- targeted therapy --- psychosocial support --- G protein–coupled receptors --- cholecystokinin --- gastrin --- gastrin-releasing peptide --- bombesin --- neurokinin --- neurotensin --- somatostatin --- circulating tumor cells --- colorectal carcinoma --- CAM invasion assay --- phenotypic mosaics --- tumor progenitor --- biomarker --- gastrointestinal malignancies --- immunotherapy --- n/a

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