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Analysis of Peptides and Proteins by Electrophoretic Techniques

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ISBN: 9783039212279 9783039212286 Year: Pages: 110 DOI: 10.3390/books978-3-03921-228-6 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Chemistry (General)
Added to DOAB on : 2019-08-28 11:21:27
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The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.

Stem Cell and Biologic Scaffold Engineering

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ISBN: 9783039214976 9783039214983 Year: Pages: 110 DOI: 10.3390/books978-3-03921-498-3 Language: English
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Biology
Added to DOAB on : 2019-12-09 11:49:15
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Tissue engineering and regenerative medicine is a rapidly evolving research field which effectively combines stem cells and biologic scaffolds in order to replace damaged tissues. Biologic scaffolds can be produced through the removal of resident cellular populations using several tissue engineering approaches, such as the decellularization method. Indeed, the decellularization method aims to develop a cell-free biologic scaffold while keeping the extracellular matrix (ECM) intact. Furthermore, biologic scaffolds have been investigated for their in vitro potential for whole organ development. Currently, clinical products composed of decellularized matrices, such as pericardium, urinary bladder, small intestine, heart valves, nerve conduits, trachea, and vessels, are being evaluated for use in human clinical trials. Tissue engineering strategies require the interaction of biologic scaffolds with cellular populations. Among them, stem cells are characterized by unlimited cell division, self-renewal, and differentiation potential, distinguishing themselves as a frontline source for the repopulation of decellularized matrices and scaffolds. Under this scheme, stem cells can be isolated from patients, expanded under good manufacturing practices (GMPs), used for the repopulation of biologic scaffolds and, finally, returned to the patient. The interaction between scaffolds and stem cells is thought to be crucial for their infiltration, adhesion, and differentiation into specific cell types. In addition, biomedical devices such as bioreactors contribute to the uniform repopulation of scaffolds. Until now, remarkable efforts have been made by the scientific society in order to establish the proper repopulation conditions of decellularized matrices and scaffolds. However, parameters such as stem cell number, in vitro cultivation conditions, and specific growth media composition need further evaluation. The ultimate goal is the development of “artificial” tissues similar to native ones, which is achieved by properly combining stem cells and biologic scaffolds and thus bringing them one step closer to personalized medicine. The original research articles and comprehensive reviews in this Special Issue deal with the use of stem cells and biologic scaffolds that utilize state-of-the-art tissue engineering and regenerative medicine approaches.

Sustainable Cropping Systems

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ISBN: 9783039289073 / 9783039289080 Year: Pages: 326 DOI: 10.3390/books978-3-03928-908-0 Language: eng
Publisher: MDPI - Multidisciplinary Digital Publishing Institute
Subject: Science (General) --- Biology --- Agriculture (General)
Added to DOAB on : 2020-06-09 16:38:57
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Global crop production must substantially increase to meet the needs of a rapidly growing population. This is constrained by the availability of nutrients, water, and land. There is also an urgent need to reduce the negative environmental impacts of crop production. Collectively, these issues represent one of the greatest challenges of the twenty-first century. Sustainable cropping systems based on ecological principles are the core of integrated approaches to solve this critical challenge. This special issue provides an international basis for revealing the underlying mechanisms of sustainable cropping systems to drive agronomic innovations. It includes review and original research articles that report novel scientific findings on improvement in cropping systems related to crop yields and their resistance to biotic and abiotic stressors, resource use efficiency, environmental impact, sustainability, and ecosystem services.

Keywords

organic cropping system --- maize --- soybean --- wheat --- partial returns --- Zea mais L. --- Triticum aestivum L. --- Helianthus annuus L. --- organic fertilization --- mineral N fertilization --- protein crops --- systematic review --- Europe --- multiple correspondence analysis (MCA) --- potato (Solanum tuberosum) --- shade --- light --- yield --- growth --- quality --- cover crops --- agrobiodiversity --- conventionalization --- system approach --- harvesting strategies --- forage yield and quality --- forage sorghum --- pearl millet --- Texas High Plains --- kura clover --- living mulch --- cover crop --- perennial --- conservation --- nitrogen --- forage --- economics --- farmer’s perception --- maize --- push-pull technology --- stemborer --- no-tillage --- conservation agriculture --- durum wheat --- gluten fractions --- SDS-PAGE analysis --- leguminous cover crop --- vetch --- double cropping --- grain yield --- N uptake --- N use efficiency --- rice --- hierarchical patch dynamics --- cropping system design --- up-scaling --- vineyard system --- complexity --- organization --- cropping systems --- water --- nitrogen --- WHCNS --- scenario analyses --- maize production --- nitrogen use efficiency --- nitrogen nutrition --- Acidic soil --- crop rotation --- enzyme activities --- green manure --- sustainable yield index --- nutrient balance --- crop residue incorporation --- straw decomposition --- residue C and N release --- SOC and STN stocks --- cover crop --- manure --- nitrate --- nitrogen --- cereal rye --- maize --- no-tillage --- cover crop --- irrigation --- weed suppression --- gross margin --- faba bean --- forage pea --- fall grazing --- cover crop --- catch crop --- nutrient cycling --- cropping systems --- sustainable crop production --- agroecology --- nutrient use efficiency --- water use efficiency --- environmental quality

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