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Sensitive assays are often based on molecular recognition. Often stability problems have limited the use of such assays. The introduction of molecularly imprinted polymers (MIPs) eliminates such limitations. With the introduction of biosensors combined with MIPs, one gets a strong combination.This volume presents how MIPs are being produced, how MIPs are integrated in different sensor concepts and finally highlights some results in certain applications.
Biosensors --- Molecularly imprinted polymers --- Biorecognition elements
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The possibility to integrate biorecognition elements into electrochemical detection systems has opened the way to a new class of powerful analytical devices named electrochemical (EC) biosensors. The first EC biosensors employed enzymes as recognition elements; however this limited their application to redox enzymes and natural or artificial redox substrates or inhibitors. Broadening this to include non-electroactive analytes was later possible thanks to the development of affinity sensors in which specific interactions between biomolecules are exploited for developing highly selective and sensitive biosensors. Presently, the combination of the exceptional molecular recognition capabilities of antibodies and aptamers with the sensitivity, low cost, practicality of use and handiness of electrochemical devices is leading to an impressive development of EC immunosensors and aptasensors that are potentially suitable to detect a wide range of analytes, following a path that is moving alongside the most recent advances in proteomics. Interestingly, with continued improvements and refinements in EC immunosensors based on the use of labels, together with intrinsically electroactive, or those with the ability to interact with electroactive molecules, a new generation of label-free sensors is being developed. This Special Issue takes stock of the state of the art and identifies prospects for EC immuno- and aptasensors, both labeled and label-free. Emphasis is placed on analytical applications for the rapid detection of disease markers and for toxicological and food analyses.
Electrochemical sensors --- Biosensors --- Immuno-test --- Aptamers --- Protein detection --- Voltammetry --- Amperometry --- Electrochemical impedance --- Functional electrodes
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ca. 200 words; this text will present the book in all promotional forms (e.g. flyers). Please describe the book in straightforward and consumer-friendly terms.[Selective and quantitative detection of different kinds of biocomponents plays an important role in biomedical applications, clinical diagnostics, environmental monitoring, toxicology, regenerative medicine and drug delivery. Therefore, multidisciplinary area of magnetic biosensing have been extensively developed in recent years, aiming to create compact analytical devices for non-expensive and low time consuming analysis provided at the point of care by non-skilled personnel. Biological samples exhibit very low magnetic background, and thus highly sensitive measurements of magnetic labels or magnetic nanoparticles enriched units can be performed without further processing. A magnetic biosensor is a compact analytical device in which magnetic transducer converts a magnetic field variation into a change of frequency, current, voltage, etc. Different types of magnetic effects are capable of creating magnetic biosensors with extra high sensitivity. This book describes interesting examples of magnetic materials based biosensors, including the synthesis of model materials for biosensor development, new engineering solutions and theoretical contributions on the magnetic biosensor sensitivity. Book contains 13 research works representing international multidisciplinary teams from Austria, China, Germany, Greece, Iran, Russia, Serbia, Spain, Taiwan and United States of America. It can be useful for PHD students and researches working in the field of magnetic nanomaterials and biomedical applications.
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Based on physiological data, intelligent algorithms can assist with the classification and recognition of the most severely impaired victims. This book presents a new sensorbased triage platform with the main proposal to join different sensor and communications technologies into a portable device. This new device must be able to assist the rescue units along with the tactical planning of the operation. This work discusses the implementation and the evaluation of the platform.
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This Special Issue aims to provide a comprehensive overview of state-of-the-art sensors technology in France. It includes research articles that consolidate our understanding of the state-of-the-art in this area and also four reviews on hot fields in sensor technology (nanomaterials, electronic tongue and optical fibre networks).
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Nucleic acids (RNA and DNA) and their chemical analogs have been utilized as building materials due to their biocompatibility and programmability. RNA, which naturally possesses a wide range of different functions, is now being widely investigated for its role as a responsive biomaterial which dynamically reacts to changes in the surrounding environment. It is now evident that artificially designed self-assembling RNAs, that can form programmable nanoparticles and supra-assemblies, will play an increasingly important part in a diverse range of applications, such as macromolecular therapies, drug delivery systems, biosensing, tissue engineering, programmable scaffolds for material organization, logic gates, and soft actuators, to name but a few. The current exciting Special Issue comprises research highlights, short communications, research articles, and reviews that all bring together the leading scientists who are exploring a wide range of the fundamental properties of RNA and DNA nanoassemblies suitable for biomedical applications.
logic gates --- nucleic acid computing --- RNA aptamers --- RNA nanotechnology --- glioblastoma multiforme --- gene therapy --- viral vector --- non-viral vector --- gene delivery --- siRNA --- RNA aptamers --- biosensors --- live-cell imaging --- fluorogenic RNA --- riboswitch --- ribozyme --- RNA nanotechnology --- RNA self-assembly --- light-up aptamer --- RNA nanoparticle --- DNA nanotechnology --- nanopores --- biological media --- serum --- stability --- aggregation --- RNA nanotechnology --- aptamers --- cotranscriptional folding --- suicide gene therapy --- non-viral gene delivery --- ganciclovir --- spinal cord tumor --- nucleic acid nanoparticles --- NANPs --- immunostimulation --- dynamic --- conditionally activated --- RNA interference --- RNA nanotechnology --- silver nanoclusters --- fluorescence --- i-motif DNA --- cytosine rich sequences --- RNA --- RNA logic --- conditional activation --- functional RNA --- nucleic acid therapeutic --- small-angle X-ray scattering --- small-angle neutron scattering --- contrast variation --- nucleic acid nanoparticle --- structural characterization --- n/a
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Phages have shown a high biotechnological potential with numerous applications. The advent of high-resolution microscopy techniques aligned with omic and molecular tools have revealed innovative phage features and enabled new processes that can be further exploited for biotechnological applications in a wide variety of fields. The high-quality original articles and reviews presented in this Special Issue demonstrate the incredible potential of phages and their derived proteins in a wide range of biotechnological applications for human benefit. Considering the emergence of amazing new available bioengineering tools and the high abundance of phages and the multitude of phage proteins yet to be discovered and studied, we believe that the upcoming years will present us with many more fascinating and new previously unimagined phage-based biotechnological applications.
gene expression regulation --- molecular probe --- macromolecular interactions --- phage-host interaction --- bacteriophage --- endolysin --- Clostridium perfringens --- alpha-sheet --- cancerous tumors --- capsid dynamics --- drug delivery vehicles --- native gel electrophoresis --- neurodegenerative disease --- pathogenic viruses --- phage display --- landscape phage --- major coat protein --- nanomedicine --- diagnostics --- biosensors --- M13 bacteriophage --- biofilm --- porous structure --- filters --- self-assembly --- T7phage library --- sarcoidosis --- tuberculosis --- microarray --- immunoscreening --- R-type pyocin --- bacteriocin --- contractile injection systems --- Pseudomonas aeruginosa --- X-ray crystallography --- receptor-binding protein --- Shigella flexneri --- bacteriophage --- tailspike proteins --- O-antigen --- serotyping --- microtiter plate assay --- fluorescence sensor --- bacteriophages --- encapsulation --- niosomes --- transfersomes --- liposomes --- Staphylococcus aureus --- phage --- Enterococcus faecalis --- Streptococcus agalactiae --- culture enrichment --- bacteriophage --- diagnostics --- Listeria monocytogenes --- endolysin --- magnetic separation --- reporter phage --- endolysin --- Pal --- Cpl-1 --- safety --- toxicity --- immune response --- Streptococcus pneumoniae --- self-assembly --- nanotubular structures --- tail sheath protein --- bacteriophage vB_EcoM_FV3 --- Appelmans --- bacteriophage evolution --- bacteriophage recombination --- phage therapy --- Pseudomonas aeruginosa --- antibiotic resistance --- bacteriophages --- Myoviridae --- bacteriophage-derived lytic enzyme --- enzybiotics --- endolysin --- in vitro activity --- ESKAPE --- n/a
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According to a report from the U.S. Centers for Disease Control and Prevention (CDC), achieving safe and healthier foods was one of the top ten achievements of public health in the 20th century. However, considerable persisting challenges currently exist in developed nations and developing economies for further assuring the safety and security of the food supplies. According to CDC estimates, as many as 3000 American adults, as an example, and based on a recent epidemiological estimate of the World Health Organization, around 420,000 individuals around the globe, lose their lives annually due to foodborne diseases. This emphasizes the need for innovative and emerging interventions, for further prevention or mitigation of the risk of foodborne microbial pathogens during food processing and manufacturing. The current publication discusses recent advancements and progress in the elimination and decontamination of microbial pathogens during various stages of manufacturing and production. Special emphasis is placed on hurdle validation studies, investigating decontamination of non-typhoidal Salmonella enterica serovars, various serogroups of Shiga toxin-producing Escherichia coli, public health-significant serotypes of Listeria monocytogenes, and pathogenic species of Cronobacter.
Listeria monocytogenes --- natural background microflora --- raw milk --- high-pressure pasteurization --- synergism of mild heat and pressure --- postharvest diseases --- food borne pathogens --- bacteria --- fungi --- food safety --- plant extracts --- small fruits --- grape --- strawberry --- blueberry --- raspberry --- blackberry --- essential oils --- Escherichia coli (STEC) --- beef --- serogroups --- stx-genes --- stx-subtypes --- Cronobacter sakazakii --- powdered infant formula --- Cronobacter outbreaks --- preventive measures --- infant care setting --- Shiga toxin-producing Escherichia coli (STEC) --- biofilm formation --- temperature --- stainless steel --- Listeria monocytogenes --- ozon --- ozonated water --- non-ozonated water --- disinfectants --- biocidal effectiveness --- Shiga toxin-producing Escherichia coli --- habituation --- carvacrol --- caprylic acid --- high-pressure pasteurization --- high hydrostatic pressure --- carbon dioxide --- nitrogen --- modified atmosphere packaging --- Escherichia coli --- dietary bioactive components --- salmonellosis --- bile acids --- epithelial barrier --- gut microbiota --- foodborne pathogens --- microfluidic chip --- rapid detection --- food safety --- biosensors --- n/a
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Mycotoxins are considered the most frequently occurring natural contaminants in human and animal diets. Considering their potential toxic and carcinogenic effects, mycotoxin exposure assessment has particular importance in the context of health risk assessment. The magnitude of a given exposure allows the derivation of the associated risk and the potential for the establishment of a disease. Although food ingestion is considered a major route of human exposure to mycotoxins, other contexts may also result in exposure, such as specific occupational environments where exposure to organic dust also occurs due to the handling of organic materials. Animals could be exposed to mycotoxins through consumption of contaminated feed, subsequently entering in the food chain and thus constituting a source of exposure to humans. Human biomonitoring is considered a new frontier for the establishment of the human internal exposure to mycotoxins. Although several studies have summarized the potential outcomes associated with mycotoxin exposure, major gaps in data remain in recognizing the mycotoxins that are the cause of diseases. This book contributes provides research that supports the anticipation of potential consequences of the exposure of humans and animals to mycotoxins, future risk assessments, and the establishment of preventive measures.
Aflatoxin B1 --- Lactobacillus casei Shirota --- Alloprevotella --- metagenomic sequencing --- microbiota --- Poultry --- Turkey --- Transcriptome --- Aflatoxin B1 --- Cecal Tonsil --- Cecum --- RNAseq --- mycotoxins --- occupational exposure --- swine production --- biomonitoring --- mycotoxins mixture --- modified HSCAS --- absorption --- T-2 toxin --- broilers --- zearalenone --- doses --- intestinal microbiome --- intestinal mycobiome --- pre-pubertal gilts --- Fusarium mycotoxins co-contamination --- ochratoxin A --- feed prevalence and safety --- HPLC analysis --- lab-on-chip --- optical biosensors --- Fab’ --- Aflatoxin M1 --- asymmetric Mach–Zehnder interferometer --- limit of detection --- affinity --- risk assessment --- total diet study --- aflatoxin B1 --- ochratoxin A --- fumonisins --- children --- Vietnam --- fumonisin B1 --- piglet --- liver --- lipids --- blood serum --- oxidation --- clinical chemistry --- histopathology --- phospholipids --- triiodothyronine --- HT-2 toxin --- cytotoxicity --- Kashin-Beck disease --- ochratoxin A --- mitigation --- mycotoxin binding --- yeast cell wall extracts --- modelling --- mycotoxins --- food consumption --- urinary biomarkers --- public health --- n/a
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