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Protocols for Pre-Field Screening of Mutants for Salt Tolerance in Rice, Wheat and Barley

Authors: --- --- --- --- et al.
ISBN: 9783319265889 9783319265902 Year: Pages: 37 DOI: 10.1007/978-3-319-26590-2 Language: English
Publisher: Springer
Subject: Agriculture (General) --- Plant Sciences
Added to DOAB on : 2017-02-15 18:41:26
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Abstract

This book offers effective, low-cost and user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents simple methods for measuring soil salinity, including soil sampling and the analysis of water-soluble salts, and describes a detailed, but simple, screening test for salt tolerance in rice, wheat and barley seedlings, which uses hydroponics. The protocols are devised for use by plant breeders and can be easily accommodated into breeding practice.

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Agriculture --- Plant

Biotechnologies for Plant Mutation Breeding: Protocols

Authors: --- --- ---
ISBN: 9783319450193 9783319450216 Year: Pages: 340 DOI: 10.1007/978-3-319-45021-6 Language: English
Publisher: Springer
Subject: Manufactures --- Chemical Engineering --- Biotechnology --- Therapeutics
Added to DOAB on : 2017-03-08 13:33:36
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This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

Authors: --- --- --- --- et al.
ISBN: 9783319162584 9783319162591 Year: Pages: 35 DOI: 10.1007/978-3-319-16259-1 Language: English
Publisher: Springer
Subject: Biotechnology --- Plant Sciences --- Manufactures --- Chemical Engineering --- Pharmacy and materia medica
Added to DOAB on : 2016-04-12 17:07:01
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Open Access provided with a grant from the International Atomic Energy Agency.This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

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